Immunoblot Assay is Still Useful For the Serological Diagnosis of Autoimmune Bullous Dermatosis

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Immunoblot Assay is Still Useful For the Serological Diagnosis of Autoimmune Bullous Dermatosis

The detection of autoantibodies to epidermal or basement membrane zone proteins by immunoblot (IB) is useful for the diagnosis and the classification of autoimmune bullous diseases (AIBD). IB using human skin extracts is actually the reference method but A431 cell line is proposed as easier alternate antigen source. We explored the performances of “A431 IB” in comparison with the reference tech...

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Parpose: Direct immunofluorescence (DIF) study on the conjunctiva is currently the classical diagnostic tool used fo the. diagnosis of autoimmune cicatrizing conjunctivitis, which is characterized by the deposition of immunoglobuliis and/or compleme:nt along the conjunctival basement membrane zone (BMZ). To diagnose the type of autoimmune conjunctivitis, we performed d&t immunoeleclron microsco...

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Quality of life index in autoimmune bullous dermatosis patients*

BACKGROUND Autoimmune bullous dermatoses are complex diseases triggered by autoantibodies action against epidermal antigens or the dermoepidermal junction. Blisters and vesicles that evolve with erosion areas characterize them. Although rare, they present high morbidity, affecting the quality of life of patients. OBJECTIVES To assess the magnitude of autoimmune bullous dermatoses on life qual...

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Clinical Relevance of Autoantibodies in Patients with Autoimmune Bullous Dermatosis

The authors present their experience related to the diagnosis, treatment, and followup of 431 patients with bullous pemphigoid, 14 patients with juvenile bullous pemphigoid, and 273 patients with pemphigus. The detection of autoantibodies plays an outstanding role in the diagnosis and differential diagnosis. Paraneoplastic pemphigoid is suggested to be a distinct entity from the group of bullou...

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Biochip Technology for the Serological Diagnosis of Bullous Pemphigoid

Bullous pemphigoid is an autoimmune blistering skin disease characterized by the presence of circulating autoantibodies which recognize specific proteins of the epidermis and dermoepidermal junction. Diagnosis is based on clinical criteria and laboratory investigations, notably histology, direct and indirect immunofluorescence, and ELISA. This study describes a new immunofluorescence assay for ...

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ژورنال

عنوان ژورنال: The Open Autoimmunity Journal

سال: 2012

ISSN: 1876-8946

DOI: 10.2174/1876894601204010001